Baicalin Attenuated PANX-1/P2X7 Axis, P2Y6, and NLRP3/Caspase-1 Signaling Pathways in Peritonitis Induced by Glaesserella parasuis

Glaesserella parasuis (G. parasuis) can cause peritonitis in piglets. However, the pathogenesis of remains unclear. Baicalin has been shown to possess anti-inflammatory and anti-oxidant functions. The aim this study was investigate role PANX-1/P2X7 axis P2Y6 signaling pathway induced by G. effect baicain on activation triggered parasuis. A serovar 5 isolate SH0165 strain obtained from lungs commercially produced pigs which had typical symptoms Glässer’s disease, namely arthritis, fibrinous polyserositis, hemorrhagic pneumonia, meningitis. Then, 35 piglets were randomly divided into five groups, each group containing seven groups consisted a negative control group, an infection 25 mg/kg baicalin 50 100 group. results showed that could promote activation; induce NLRP3/caspase-1, IL-1β IL-18 expression; trigger PLC/PKC MLCK/MLC attenuate expression tight junction proteins ZO-1, E-cadherin, Occludins, claudin 1; stimulate CD14, CD24, CD36, CD47, CD91 peritoneum as measured via Western blot (p < 0.01; PLC, p 0.05). significantly inhibit axis, P2Y6, NLRP3/caspase-1 reduce occludins, 1 blot. Our deepen understanding mechanism provide some novel potential methods controlling infection.